Journal: Cell

Article Title: DE NOVO EPIGENETIC PROGRAMS INHIBIT PD-1 BLOCKADE-MEDIATED T-CELL REJUVENATION

doi: 10.1016/j.cell.2017.06.007

Figure Lengend Snippet: Key Resources Table

Article Snippet: Anti-mouse/human Tcf1 (clone C63D9) , Cell Signaling , Cat#14456S.

Techniques: Virus, Recombinant, DNA Methylation Assay, Plasmid Preparation, Cloning, cDNA Synthesis, Microarray, Software, Methylation Sequencing, Flow Cytometry

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Inflammation Induces Lipid Deposition in Kidneys by Downregulating Renal PCSK9 in Mice with Adriamycin-Induced Nephropathy

doi: 10.12659/MSM.917312

Figure Lengend Snippet: ( A–F ) Renal expression of TGF-β1, IL-1β, HNF1α, PCSK9, LDLR, and SREBP2 proteins (immunohistochemical analysis software was used to analyze the optical density values in the images, and semi-quantitative values of positive expression in kidney tissues were obtained) and mRNAs (from RT-qPCR, relative to GAPDH) in the 4 groups at 4, 8, and 12 weeks. Values represent means ± standard errors of mean for groups of 8 mice each. For comparisons at the same time point: * P <0.05 versus CTL, # P <0.05 versus AWI. TGF-β1 – transforming growth factor beta 1; IL-1β – interleukin 1β; HNF1α – hepatocyte nuclear factor 1α; PCSK9 – pro-protein convertase subtilisin kexin type 9; LDLR – low-density lipoprotein receptor; SREBP2 – sterol regulatory element binding protein 2; RT-q-PCR – real-time quantitative polymerase chain reaction; GAPDH – glyceraldehyde 3-phosphate dehydrogenase; CLT – control group; AWI group – Adriamycin-induced nephrosis with inflammation group.

Article Snippet: Immunohistochemistry (IHC) was used to examine the expression of interleukin (IL)-1β, transforming growth factor beta 1 (TGF-β1), HNF1α, PCSK9, LDLR, and SREBP2 using the following antibodies: rabbit anti-rat IL-1β antibody (working dilution 1: 100; sc-7884, Santa Cruz Biotechnology, USA), rabbit anti-rat TGF-β1 (working dilution 1: 100; sc-146, Santa Cruz Biotechnology), mouse anti-human SREBP2 antibody (working dilution 1: 100; sc-271615, Santa Cruz Biotechnology), mouse anti-human HNF1 α antibody (working dilution 1: 100; sc-393668, Santa Cruz Biotechnology,), rabbit anti-rat LDLR antibody (working dilution 1: 100; ab30532, Abcam, UK), rabbit anti-rat PCSK9 antibody (working dilution 1: 100; ab31762, Abcam).

Techniques: Expressing, Immunohistochemical staining, Software, Quantitative RT-PCR, Binding Assay, Real-time Polymerase Chain Reaction, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Inflammation Induces Lipid Deposition in Kidneys by Downregulating Renal PCSK9 in Mice with Adriamycin-Induced Nephropathy

doi: 10.12659/MSM.917312

Figure Lengend Snippet: Representative immunostaining results of renal tissues for HNF1α, PCSK9, LDLR, and SREBP2 in each group at 4, 8, and 12 weeks. Scale bars indicate 100 μm. HNF1α – hepatocyte nuclear factor 1α; PCSK9 – pro-protein convertase subtilisin kexin type 9; LDLR – low-density lipoprotein receptor; SREBP2 – sterol regulatory element binding protein 2.

Article Snippet: Immunohistochemistry (IHC) was used to examine the expression of interleukin (IL)-1β, transforming growth factor beta 1 (TGF-β1), HNF1α, PCSK9, LDLR, and SREBP2 using the following antibodies: rabbit anti-rat IL-1β antibody (working dilution 1: 100; sc-7884, Santa Cruz Biotechnology, USA), rabbit anti-rat TGF-β1 (working dilution 1: 100; sc-146, Santa Cruz Biotechnology), mouse anti-human SREBP2 antibody (working dilution 1: 100; sc-271615, Santa Cruz Biotechnology), mouse anti-human HNF1 α antibody (working dilution 1: 100; sc-393668, Santa Cruz Biotechnology,), rabbit anti-rat LDLR antibody (working dilution 1: 100; ab30532, Abcam, UK), rabbit anti-rat PCSK9 antibody (working dilution 1: 100; ab31762, Abcam).

Techniques: Immunostaining, Binding Assay

Journal: iScience

Article Title: Exogenous IL-2 delays memory precursors generation and is essential for enhancing memory cells effector functions

doi: 10.1016/j.isci.2024.109411

Figure Lengend Snippet: CD8 + T cells activated in the presence of ex-IL-2 display stronger effector functions See also Figure S6 . 1.5 × 10 5 magnetically purified naive F5 CD8 + T cells labeled with CTV were activated with CpG-matured, NP68-loaded cDC at a ratio of cDC:CD8 = 1:10, in the presence or absence of ex-IL-2 (11,5 ng/mL). (A) Median Fluorescence Intensity (MFI) of TCF1 was measured on divided cells after 4 days of activation and the ratio between cells cultured in the absence and cells cultured in the presence of ex-IL-2 was calculated. (B and C) After 3, 4, or 5 days of activation, CD8 + T cells were restimulated for 2h with NP68 and the percentages of IFN-γ- (B) and GzmB- (C Left) expressing CD8 + T cells were measured on divided cells. Representative dot plots and individual percentages of GzmB + cells are also depicted (C Right). (D) Percentages of live EL4 target cells, loaded or not with NP68 peptide, after 4h of co-culture with effector CD8 + T cells activated for 5 days. Values from three independent experiments are presented. The results are expressed as the mean +SD. E:T ratio = effector:target ratio. The mean ± SD of six independent experiments is shown in panel A. The mean ± SEM of triplicate cultures from one representative experiment out of three independent experiments is shown in panel B and C (right). In A, the statistical significance of the difference between the mean value of ratios and the hypothetical value of 1 was determined by the one sample t-test. In D, the statistical significance of differences between F5 CD8 + cultured with or without ex-IL-2 at each E:T ratio was assessed by a two-way ANOVA followed by Sidak’s multiple comparison test (ns = p > 0.05, ∗∗ = p ≤ 0.01 and ∗∗∗ = p ≤ 0.001).

Article Snippet: PE anti-mouse TCF1 (clone S33-966) , BD Biosciences , Cat#564217 (AB_2687845).

Techniques: Purification, Labeling, Fluorescence, Activation Assay, Cell Culture, Expressing, Co-Culture Assay, Comparison

Journal: iScience

Article Title: Exogenous IL-2 delays memory precursors generation and is essential for enhancing memory cells effector functions

doi: 10.1016/j.isci.2024.109411

Figure Lengend Snippet:

Article Snippet: PE anti-mouse TCF1 (clone S33-966) , BD Biosciences , Cat#564217 (AB_2687845).

Techniques: Virus, Modification, Western Blot, Recombinant, Cell Isolation, Staining, Enzyme-linked Immunosorbent Assay, Software

Journal: Immunity

Article Title: TCR-independent CD137 (4–1BB) signaling promotes CD8 + -exhausted T cell proliferation and terminal differentiation

doi: 10.1016/j.immuni.2023.06.007

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-human/mouse Tcf1/tcf7 AF488 [C63D9] , Cell Signaling Technology , Cat#: 6444S.

Techniques: Purification, Recombinant, Lysis, Negative Control, Cell Isolation, Staining, Knock-Out, Isolation, Control, Infection, RNA Sequencing, Software, Gene Expression